Wacky Western TEM Work

Two Friday’s ago, half of the Phage class took to the road to do some TEM work on our mycobacteriophage samples. Having been in the lab a lot and doing computer work, it was fun to get out to a new location with people we have been familiar with all year.

It was actually a really fun day, and if you get the chance to check Twitter, search for my hashtag #phaginghard and you’ll find a stream of tweets from the hilarious things that were said throughout the day.

If you’re not familiar with TEM, its a Transmission electron Microscope. It takes up a small room and works as a beam of electrons is transmitted through a thin specimen. After putting our phage lysates on a thin copper mesh circle (that is really tiny), we loaded our discs into the apparatus and got some cutesy little pictures of our phage! My sample only had four phage, so the microscope operator was giving me a hard time about causing him a lot of work ;).

Each imaging session took around 30 minutes, so while each student was in getting their photos, the rest of us were studying for a big biology test and playing Euchre. Overall, it was a great time had by all, and we got a bit closer to our phage associates.

I hope you all had a great Easter break!

More soon,

A

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Amanda Porter

Hi! I'm Amanda. I'm from Traverse City, Mi and I'm a Junior Biochemistry and Molecular Biology major. I do research for the Fraley lab on the gut bacterial ecology of Pekin ducks and I work for the Chemistry department! Aspiring Neurosurgeon and LOVE to learn in all situations. Follow me on Twitter! @hopeamanda15

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